University of Puerto Rico

Río Piedras Campus

College of Natural Sciences

Nutrition and Dietetics Program

Program: Bachelor of Science in

Nutrition and Dietetics


Human Biochemistry Laboratory, NUTR  4159


First Semester, 2012-2013 Academic Year

Sec. 0U1, Wednesday, 1:00 p.m. – 3:50 p.m.

Room NEP 577


Michelle Schelske Santos, Ph.D.; This email address is being protected from spambots. You need JavaScript enabled to view it.,  This email address is being protected from spambots. You need JavaScript enabled to view it.; ext. 5798, 2792





Academic Recess – Tropical Storm Isaac




LAB: Syllabus, Preparation of LAB Notebooks

LAB Excel workshop – Data Tables and Graphs;

Register for course in Edu 2.0

Introduction to course & prep of Lab Notebook

Introduction to Excel for Lab Reports--Water content of foods


General Laboratory Safety; Equipment assignment Intro to instrumentation; (bring lab coats and lab notebooks)

Quiz in Edu2.0; open 24h before lab

General Lab Safety; Introduction to Scientific Instrumentation



LAB Safety (Biohazard)

Post-conference written quiz; practical lab quiz

Micropipette and Pipette Techniques; practical lab quiz

Blood-borne Pathogens and Biohazard Disposal (OPASO; Dr. Warner Ithier) Micropipette and Pipetting Techniques



LAB – DNA Extraction

LAB – Tissue Homogenation of Protein Samples

Quiz in Edu 2.0; open 24h before lab

DNA Extraction from strawberries

Homogenation of animal & vegetable protein


LAB Computer Workshop – Info Databases and your Search Strategy (Biblioteca Ciencias Naturales; Prof. Pura Centeno)

Assign.: Search Strategies, DNA & Protein Methods

Biochemical methods for DNA and protein analyses in the field of nutrition



LAB – Total Protein Determination

Quiz in Edu 2.0; open 24h before lab

Due: Assignment Search Strategies DNA & Protein Method

Protein content of animal versus vegetable protein on microscale using microplate reader;

Comparison of protein content of human milk, cow milk and infant formula



LAB Expt: Active Acidity and pH;

Quiz in Edu2.0 open 24h before lab

Active Acidity and pH



LAB: Protein Electrophoresis of blood fractions I

Quiz in Edu 2.0; open 24h before lab

Preparation of blood fractions

Preparation for and dry run of electrophoresis


LAB: Protein Electrophoresis of blood fractions II

Quiz in Edu 2.0; open 24h before lab

Protein electrophoresis, gel staining & drying


LAB OP1 – (50 pts.; 1h)

Bioinformatics Assignment

Experiments to date, including protein electro.

Use of experimental and stored data



Academic Recess - Elections



LAB: Expt. 30, Vitamin C

Quiz in Edu 2.0; open 24h before lab

Due Bioinformatics (Edu 2.0)

Colorimetric Determination of Vitamin C: Raw vs. Processed Foods


LAB – Expt. Membrane Structure and Function; Quiz in Blackboard; open 24h before lab

Due SDS-PAGE Lab (in notebook)

Blood Typing

Digital Microscopy of Blood Cells


Extraction of DNA from whole blood (TBconfirmed)


Extraction of DNA from student leucocytes


Electrophoresis of DNA

Horizontal agarose gel electrophoresis of nucleic acids



LAB – Lipids and Fiber

Quiz in Edu 2.0; open 24h before lab

Dietary Lipids, Fiber & Fattaché

TBA, Dec. 17-21, 2012

Lab OP 2 – (50 pts.; 1h)

Due: Lab Notebooks

(Experiments from second half of semester)




LAB: Glycolytic Fermentation

Anaerobic fermentation of sugars by yeast

Anaerobic fermentation of glucose by muscle


Oral Presentations & Discussion of assigned lab procedures, results, and conclusions or related topics

Due: Written assignment of topic

Includes all previous lab experiences and related topics


This schedule is subject to change based on unforeseen events, natural disasters, scheduling of events, and changes in laboratory equipment and material arrival dates.  In the event of any change, a new schedule will be developed and available in Edu 2.0.


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