University of Puerto Rico

Río Piedras Campus

College of Natural Sciences

Nutrition and Dietetics Program

Program: Bachelor of Science in

Nutrition and Dietetics

 

Human Biochemistry Laboratory, NUTR  4159

 

First Semester, 2012-2013 Academic Year

Sec. 0U1, Wednesday, 1:00 p.m. – 3:50 p.m.

Room NEP 577

 

Michelle Schelske Santos, Ph.D.; This email address is being protected from spambots. You need JavaScript enabled to view it.,  This email address is being protected from spambots. You need JavaScript enabled to view it.; ext. 5798, 2792

Date

Assignment

Topic

22Aug12

Academic Recess – Tropical Storm Isaac

 

 

29Aug12

LAB: Syllabus, Preparation of LAB Notebooks

LAB Excel workshop – Data Tables and Graphs;

Register for course in Edu 2.0

Introduction to course & prep of Lab Notebook

Introduction to Excel for Lab Reports--Water content of foods

05Sep12

General Laboratory Safety; Equipment assignment Intro to instrumentation; (bring lab coats and lab notebooks)

Quiz in Edu2.0; open 24h before lab

General Lab Safety; Introduction to Scientific Instrumentation

 

12Sep12

LAB Safety (Biohazard)

Post-conference written quiz; practical lab quiz

Micropipette and Pipette Techniques; practical lab quiz

Blood-borne Pathogens and Biohazard Disposal (OPASO; Dr. Warner Ithier) Micropipette and Pipetting Techniques

 

19Sep12

LAB – DNA Extraction

LAB – Tissue Homogenation of Protein Samples

Quiz in Edu 2.0; open 24h before lab

DNA Extraction from strawberries

Homogenation of animal & vegetable protein

26Sep12

LAB Computer Workshop – Info Databases and your Search Strategy (Biblioteca Ciencias Naturales; Prof. Pura Centeno)

Assign.: Search Strategies, DNA & Protein Methods

Biochemical methods for DNA and protein analyses in the field of nutrition

 

03Oct12

LAB – Total Protein Determination

Quiz in Edu 2.0; open 24h before lab

Due: Assignment Search Strategies DNA & Protein Method

Protein content of animal versus vegetable protein on microscale using microplate reader;

Comparison of protein content of human milk, cow milk and infant formula

10Oct12

 

LAB Expt: Active Acidity and pH;

Quiz in Edu2.0 open 24h before lab

Active Acidity and pH

17Oct12

 

LAB: Protein Electrophoresis of blood fractions I

Quiz in Edu 2.0; open 24h before lab

Preparation of blood fractions

Preparation for and dry run of electrophoresis

24Oct12

LAB: Protein Electrophoresis of blood fractions II

Quiz in Edu 2.0; open 24h before lab

Protein electrophoresis, gel staining & drying

31Oct12

LAB OP1 – (50 pts.; 1h)

Bioinformatics Assignment

Experiments to date, including protein electro.

Use of experimental and stored data

07Nov12

 

Academic Recess - Elections

 

14Nov12

LAB: Expt. 30, Vitamin C

Quiz in Edu 2.0; open 24h before lab

Due Bioinformatics (Edu 2.0)

Colorimetric Determination of Vitamin C: Raw vs. Processed Foods

21Nov12

LAB – Expt. Membrane Structure and Function; Quiz in Blackboard; open 24h before lab

Due SDS-PAGE Lab (in notebook)

Blood Typing

Digital Microscopy of Blood Cells

28Nov12

Extraction of DNA from whole blood (TBconfirmed)

 

Extraction of DNA from student leucocytes

05Dec12

Electrophoresis of DNA

Horizontal agarose gel electrophoresis of nucleic acids

12Dec12

(Rep.22aug12)

LAB – Lipids and Fiber

Quiz in Edu 2.0; open 24h before lab

Dietary Lipids, Fiber & Fattaché

TBA, Dec. 17-21, 2012

Lab OP 2 – (50 pts.; 1h)

Due: Lab Notebooks

(Experiments from second half of semester)

 

XXXXXX

 

LAB: Glycolytic Fermentation

Anaerobic fermentation of sugars by yeast

Anaerobic fermentation of glucose by muscle

XXXXXX

Oral Presentations & Discussion of assigned lab procedures, results, and conclusions or related topics

Due: Written assignment of topic

Includes all previous lab experiences and related topics

 

This schedule is subject to change based on unforeseen events, natural disasters, scheduling of events, and changes in laboratory equipment and material arrival dates.  In the event of any change, a new schedule will be developed and available in Edu 2.0.

 

Edited by MSS, 02oct12

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